SR-2B rabbit pAb from ELK Biotechnology Read more ›
SR-2B rabbit pAb is a research-use antibody from ELK Biotechnology for studying SR-2B in WB, IF, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.
This gene encodes one of the several different receptors for 5-hydroxytryptamine (serotonin) that belongs to the G-protein coupled receptor 1 family. Serotonin is a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. Serotonin receptors mediate many of the central and peripheral physiologic functions of serotonin, including regulation of cardiovascular functions and impulsive behavior. Population and family-based analyses of a minor allele (glutamine-to-stop substitution, designated Q20*) which blocks expression of this protein, and knockout studies in mice, suggest a role for this gene in impulsivity. However, other factors, such as elevated testosterone levels, may also be involved. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2016],
| Specificity | SR-2B Polyclonal Antibody detects endogenous levels of SR-2B protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using SR-2B Polyclonal Antibody | Immunofluorescence analysis of COS7 cells, using HTR2B Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of lysates from HT-29, 293, and HeLa cells, using HTR2B Antibody. The lane on the right is blocked with the synthesized peptide. | Western blot analysis of the lysates from COLO205 cells using HTR2B antibody. |
| Collaborator | ELK Biotechnology |
| Partner model | ES3492 |
| Target | SR-2B |
| Research areas | >>Calcium signaling pathway, >>Neuroactive ligand-receptor interaction, >>Gap junction, >>Serotonergic synapse, >>Inflammatory mediator regulation of TRP channels |
| Reactivity | Human,Rat,Mouse |
| Applications | WB,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human HTR2B. AA range:15-64 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Ubiquitous. Detected in liver, kidney, heart, pulmonary artery, and intestine. Detected at lower levels in blood, placenta and brain, especially in cerebellum, occipital cortex and frontal cortex. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. | — |
| Rat | — | — |
| Mouse | — | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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