SLK rabbit pAb
SLK rabbit pAb from ELK Biotechnology Read more ›
Product Description
SLK rabbit pAb is an ELK Biotechnology antibody for research workflows involving SLK.
Background
catalytic activity:ATP + a protein = ADP + a phosphoprotein.,function:Mediates apoptosis and actin stress fiber dissolution.,PTM:Autophosphorylated. Phosphorylated upon DNA damage, probably by ATM or ATR.,PTM:Proteolytically cleaved by caspase-3.,sequence caution:Contaminating sequence. Potential poly-A sequence starting in position 422.,sequence caution:Contaminating sequence. Potential poly-A sequence starting in position 611.,sequence caution:Contaminating sequence. Potential poly-A sequence starting in position 614.,similarity:Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 UVR domain.,tissue specificity:Ubiquitously expressed. Highest expression is found in heart and in skeletal muscle.,
Additional antibody information
| Specificity | SLK Polyclonal Antibody detects endogenous levels of SLK protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using SLK Polyclonal Antibody diluted at 1:1000 | Immunofluorescence analysis of HepG2 cells, using SLK Antibody. The picture on the right is blocked with the synthesized peptide. | Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue, using SLK Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of lysates from COLO205 cells, using SLK Antibody. The lane on the right is blocked with the synthesized peptide. |
Key Facts
| Target | SLK |
| Also known as | SLK; KIAA0204; STK2; STE20-like serine/threonine-protein kinase; STE20-like kinase; hSLK; CTCL tumor antigen se20-9; STE20-related serine/threonine-protein kinase; STE20-related kinase; Serine/threonine-protein kinase 2 |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. |
| Species reactivity | Human;Mouse;Rat |
| Observed band | 150kD |
| Cellular localization | Cytoplasm . |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Oocyte meiosis |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBIHCIFELISA |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human SLK. AA range:1151-1200 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.