Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM97665

SLC6A8 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

SLC6A8 rabbit pAb from ELK Biotechnology Read more ›

SLC6A8 rabbit pAb
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SLC6A8 rabbit pAb

Product Description

SLC6A8 rabbit pAb is a research-use antibody from ELK Biotechnology for studying SLC6A8 in WB, IHC workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

The protein encoded by this gene is a plasma membrane protein whose function is to transport creatine into and out of cells. Defects in this gene can result in X-linked creatine deficiency syndrome. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2008],

Additional antibody information

Specificity SLC6A8 Polyclonal Antibody detects endogenous levels of SLC6A8 protein.
Validation evidence captions Western Blot analysis of various cells using SLC6A8 Polyclonal Antibody | Western blot analysis of SLC6A8 Antibody. The lane on the right is blocked with the SLC6A8 peptide. | Western blot analysis of the lysates from HUVECcells using SLC6A8 antibody. | Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).

Key Facts

Product typePrimary Antibody Target: SLC6A8
Collaborator ELK Biotechnology
Partner model ES3453
Target SLC6A8
Research areasAntibody Research
Reactivity Human,Mouse,Rat
Applications WB,IHC
ConjugationUnconjugated
Dilution rangeWB 1:500-2000;IHC-p 1:50-300
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen The antiserum was produced against synthesized peptide derived from human SLC6A8. AA range:581-630
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Predominantly expressed in skeletal muscle and kidney. Also found in brain, heart, colon, testis and prostate.
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:500-2000;IHC-p 1:50-300
Mouse
Rat
ABMIUM Validated™ IHC
Species Dilution Notes

Specifications

Target
SLC6A8
Also known as
SLC6A8; Sodium- and chloride-dependent creatine transporter 1; CT1; Creatine transporter 1; Solute carrier family 6 member 8
Concentration
1 mg/ml
Working concentration
WB 1:500-2000;IHC-p 1:50-300
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat
Tested applications
WBIHC
Immunogen
The antiserum was produced against synthesized peptide derived from human SLC6A8. AA range:581-630
Observed band
70kD
Cellular localization
Membrane; Multi-pass membrane protein.
Dilution range
WB 1:500-2000;IHC-p 1:50-300
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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