Ribosomal Protein S4X rabbit pAb
Ribosomal Protein S4X rabbit pAb from ELK Biotechnology Read more ›
Product Description
Ribosomal Protein S4X rabbit pAb is an ELK Biotechnology antibody for research workflows involving Ribosomal Protein S4X.
Background
Cytoplasmic ribosomes, organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes ribosomal protein S4, a component of the 40S subunit. Ribosomal protein S4 is the only ribosomal protein known to be encoded by more than one gene, namely this gene and ribosomal protein S4, Y-linked (RPS4Y). The 2 isoforms encoded by these genes are not identical, but are functionally equivalent. Ribosomal protein S4 belongs to the S4E family of ribosomal proteins. This gene is not subject to X-inactivation. It has been suggested that haploinsufficiency of the ribosomal protein S4 genes plays a role in Turner syndrome; however, this hypothesis is controversial. As is typical for genes encoding ribosomal proteins, there are multiple processed pseud
Additional antibody information
| Specificity | Ribosomal Protein S4X Polyclonal Antibody detects endogenous levels of Ribosomal Protein S4X protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using Ribosomal Protein S4X Polyclonal Antibody diluted at 1:1000 | Western Blot analysis of customer's (cat sample) using Ribosomal Protein S4X Polyclonal Antibody diluted at 1:1000 | Immunofluorescence analysis of HUVEC cells, using RPS4X Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of lysates from HeLa and COLO cells, using RPS4X Antibody. The lane on the right is blocked with the synthesized peptide. |
Key Facts
| Target | Ribosomal Protein S4X |
| Also known as | RPS4X; CCG2; RPS4; SCAR; 40S ribosomal protein S4; X isoform; SCR10; Single copy abundant mRNA protein |
| Concentration | 1 mg/ml |
| Working concentration | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 |
| Species reactivity | Human;Mouse;Rat;Cat |
| Observed band | 30kD |
| Cellular localization | Cytoplasm . Localized in cytoplasmic mRNP granules containing untranslated mRNAs. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Ribosome;>>Coronavirus disease - COVID-19 |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBIHCELISA |
| Dilution range | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 |
| Immunogen | The antiserum was produced against synthesized peptide derived from human RPS4X. AA range:81-130 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Reactivity & Application Validation
4 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat | |||||||
| Cat |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 | — |
| Mouse | — | — |
| Rat | — | — |
| Cat | — | — |
| Species | Dilution | Notes |
|---|
| Species | Dilution | Notes |
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Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.