Ribosomal Protein S3 rabbit pAb from ELK Biotechnology Read more ›
Ribosomal Protein S3 rabbit pAb is a research-use antibody from ELK Biotechnology for studying Ribosomal Protein S3 in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit, where it forms part of the domain where translation is initiated. The protein belongs to the S3P family of ribosomal proteins. Studies of the mouse and rat proteins have demonstrated that the protein has an extraribosomal role as an endonuclease involved in the repair of UV-induced DNA damage. The protein appears to be located in both the cytoplasm and nucleus but not in the nucleolus. Higher levels of expression of this gene in colon adenocarcinomas and adenomatous polyps compared to adjacent normal colonic mucosa have been observed. This gene is co-transcribed with the small nucleolar RNA genes U15A and
| Specificity | Ribosomal Protein S3 Polyclonal Antibody detects endogenous levels of Ribosomal Protein S3 protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using Ribosomal Protein S3 Polyclonal Antibody | Immunohistochemistry analysis of paraffin-embedded human tonsil tissue, using RPS3 Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of lysates from HepG2 cells, using RPS3 Antibody. The lane on the right is blocked with the synthesized peptide. | Western blot analysis of the lysates from RAW264.7cells using RPS3 antibody. |
| Collaborator | ELK Biotechnology |
| Partner model | ES3373 |
| Target | Ribosomal Protein S3 |
| Research areas | >>Ribosome, >>Pathogenic Escherichia coli infection, >>Salmonella infection, >>Coronavirus disease - COVID-19 |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human RPS3. AA range:171-220 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Adrenal cortex,Cervix carcinoma,Colon adenocarcinoma,Epithelium,Liver,Lymph,Pituita |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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