Ribosomal Protein L30 rabbit pAb from ELK Biotechnology Read more ›
Ribosomal Protein L30 rabbit pAb is a research-use antibody from ELK Biotechnology for studying Ribosomal Protein L30 in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat, Monkey. Specifications and supporting documents below should be reviewed when planning the experiment.
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L30E family of ribosomal proteins. It is located in the cytoplasm. This gene is co-transcribed with the U72 small nucleolar RNA gene, which is located in its fourth intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008],
| Specificity | Ribosomal Protein L30 Polyclonal Antibody detects endogenous levels of Ribosomal Protein L30 protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using Ribosomal Protein L30 Polyclonal Antibody diluted at 1:2000 | Immunohistochemistry analysis of paraffin-embedded human prostate carcinoma tissue, using RPL30 Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of lysates from A549, COS7, and RAW264.7 cells, using RPL30 Antibody. The lane on the right is blocked with the synthesized peptide. | Western blot analysis of the lysates from HT-29 cells using RPL30 antibody. |
| Collaborator | ELK Biotechnology |
| Partner model | ES3364 |
| Target | Ribosomal Protein L30 |
| Research areas | >>Ribosome, >>Coronavirus disease - COVID-19 |
| Reactivity | Human,Mouse,Rat,Monkey |
| Applications | WB,IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human RPL30. AA range:66-115 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Embryonic stem cell,Epithelium,Mammary gland,Placenta, |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat | |||||||
| Monkey |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Monkey | — | — |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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