Ribosomal Protein L3 rabbit pAb
Ribosomal Protein L3 rabbit pAb from ELK Biotechnology Read more ›
Product Description
Ribosomal Protein L3 rabbit pAb is an ELK Biotechnology antibody for research workflows involving Ribosomal Protein L3.
Background
Ribosomes, the complexes that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L3P family of ribosomal proteins and it is located in the cytoplasm. The protein can bind to the HIV-1 TAR mRNA, and it has been suggested that the protein contributes to tat-mediated transactivation. This gene is co-transcribed with several small nucleolar RNA genes, which are located in several of this gene's introns. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul
Additional antibody information
| Specificity | Ribosomal Protein L3 Polyclonal Antibody detects endogenous levels of Ribosomal Protein L3 protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using Ribosomal Protein L3 Polyclonal Antibody | Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using RPL3 Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of lysates from HT-29 cells, using RPL3 Antibody. The lane on the right is blocked with the synthesized peptide. | Western blot analysis of the lysates from HUVECcells using RPL3 antibody. |
Key Facts
| Target | Ribosomal Protein L3 |
| Also known as | RPL3; OK/SW-cl.32; 60S ribosomal protein L3; HIV-1 TAR RNA-binding protein B; TARBP-B |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications. |
| Species reactivity | Human;Mouse;Rat |
| Observed band | 46kD |
| Cellular localization | Nucleus, nucleolus . Cytoplasm . |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Ribosome;>>Coronavirus disease - COVID-19 |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBIHCIFELISA |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human RPL3. AA range:211-260 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.