RhoGEF p115 rabbit pAb from ELK Biotechnology Read more ›
RhoGEF p115 rabbit pAb is a research-use antibody from ELK Biotechnology for studying RhoGEF p115 in WB, IHC, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate Rho-dependent signals. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been defined. [provided by RefSeq, Jul 2008],
| Specificity | RhoGEF p115 Polyclonal Antibody detects endogenous levels of RhoGEF p115 protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using RhoGEF p115 Polyclonal Antibody | Western blot analysis of the lysates from HepG2 cells using ARHGEF1 antibody. | Immunohistochemical analysis of paraffin-embedded human Gastric adenocarcinoma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min). |
| Collaborator | ELK Biotechnology |
| Partner model | ES3355 |
| Target | RhoGEF p115 |
| Research areas | >>Vascular smooth muscle contraction, >>Platelet activation, >>Regulation of actin cytoskeleton, >>Parathyroid hormone synthesis, secretion and action, >>Pathogenic Escherichia coli infection, >>Yersinia infection, >>Human cytomegalovirus infection, >>Pathways in cancer, >>Proteoglycans in cancer, >>Lipid and atherosclerosis |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,ELISA |
| Conjugation | Unconjugated |
| Dilution range | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human ARHGEF1. AA range:162-211 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Ubiquitously expressed. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
|---|
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
No customer reviews yet.
