RASSF4 rabbit pAb from ELK Biotechnology Read more ›
RASSF4 rabbit pAb is a research-use antibody from ELK Biotechnology for studying RASSF4 in WB, ELISA workflows with listed reactivity for Human, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
The function of this gene has not yet been determined but may involve a role in tumor suppression. Alternative splicing of this gene results in several transcript variants; however, most of the variants have not been fully described. [provided by RefSeq, Jul 2008],
| Specificity | RASSF4 Polyclonal Antibody detects endogenous levels of RASSF4 protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using RASSF4 Polyclonal Antibody | Western blot analysis of lysates from COLO and HUVEC cells, using RASSF4 Antibody. The lane on the right is blocked with the synthesized peptide. | Western blot analysis of the lysates from RAW264.7cells using RASSF4 antibody. |
| Collaborator | ELK Biotechnology |
| Partner model | ES3329 |
| Target | RASSF4 |
| Research areas | >>Hippo signaling pathway - multiple species |
| Reactivity | Human,Rat |
| Applications | WB,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human RASSF4. AA range:134-183 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Widely expressed. Frequently down-regulated in tumor cell lines. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. | — |
| Rat | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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