ABM97591
Rab 35 rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
Rab 35 rabbit pAb from ELK Biotechnology Read more ›
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Product Description
Rab 35 rabbit pAb is an ELK Biotechnology antibody for research workflows involving Rab 35.
Background
function:Possesses GTPase activity.,similarity:Belongs to the small GTPase superfamily. Rab family.,subcellular location:Identified by mass spectrometry in melanosome fractions from stage I to stage IV.,
Additional antibody information
| Specificity | Rab 35 Polyclonal Antibody detects endogenous levels of Rab 35 protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using Rab 35 Polyclonal Antibody | Western blot analysis of RAB35 Antibody. The lane on the right is blocked with the RAB35 peptide. | Western blot analysis of the lysates from HT-29 cells using RAB35 antibody. | Immunohistochemical analysis of paraffin-embedded human lung cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min). |
Key Facts
Product type
Primary Antibody›
Target: Rab 35
| Target | Rab 35 |
| Also known as | RAB35; RAB1C; RAY; Ras-related protein Rab-35; GTP-binding protein RAY; Ras-related protein Rab-1C |
| Concentration | 1 mg/ml |
| Working concentration | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 |
| Species reactivity | Human;Mouse;Rat |
| Observed band | 24kD |
| Cellular localization | Cell membrane ; Lipid-anchor ; Cytoplasmic side . Membrane, clathrin-coated pit . Cytoplasmic vesicle, clathrin-coated vesicle . Endosome . Melanosome . Present on sorting endosomes and recycling endosome tubules (PubMed:16950109). Tends to be enriched in PIP2-positive cell membrane domains (PubMed:16950109). During mitosis, associated with the plasma membrane and present at the ingressing furrow during early cytokinesis as well as at the intercellular bridge later during cytokinesis (PubMed:16950109). Identified in stage I to stage IV melanosomes (PubMed:17081065). . |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Endocytosis |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBIHCELISA |
| Dilution range | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 |
| Immunogen | The antiserum was produced against synthesized peptide derived from human RAB35. AA range:99-148 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
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Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 | — |
| Mouse | — | — |
| Rat | — | — |
ABMIUM Validated™
IHC
| Species | Dilution | Notes |
|---|
ABMIUM Validated™
ELISA
| Species | Dilution | Notes |
|---|
Specifications
Target
Rab 35
Also known as
RAB35; RAB1C; RAY; Ras-related protein Rab-35; GTP-binding protein RAY; Ras-related protein Rab-1C
Concentration
1 mg/ml
Working concentration
WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human;Mouse;Rat
Tested applications
WBIHCELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human RAB35. AA range:99-148
Observed band
24kD
Cellular localization
Cell membrane ; Lipid-anchor ; Cytoplasmic side . Membrane, clathrin-coated pit . Cytoplasmic vesicle, clathrin-coated vesicle . Endosome . Melanosome . Present on sorting endosomes and recycling endosome tubules (PubMed:16950109). Tends to be enriched in PIP2-positive cell membrane domains (PubMed:16950109). During mitosis, associated with the plasma membrane and present at the ingressing furrow during early cytokinesis as well as at the intercellular bridge later during cytokinesis (PubMed:16950109). Identified in stage I to stage IV melanosomes (PubMed:17081065). .
Dilution range
WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage & Stability
Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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