ABM97589
PUMA rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
PUMA rabbit pAb from ELK Biotechnology Read more ›
Related formats
Zoom
Product Description
PUMA rabbit pAb is an ELK Biotechnology antibody for research workflows involving PUMA.
Background
This gene encodes a member of the BCL-2 family of proteins. This family member belongs to the BH3-only pro-apoptotic subclass. The protein cooperates with direct activator proteins to induce mitochondrial outer membrane permeabilization and apoptosis. It can bind to anti-apoptotic Bcl-2 family members to induce mitochondrial dysfunction and caspase activation. Because of its pro-apoptotic role, this gene is a potential drug target for cancer therapy and for tissue injury. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2011],
Additional antibody information
| Specificity | PUMA Polyclonal Antibody detects endogenous levels of PUMA protein. |
|---|---|
| Validation evidence captions | Immunofluorescence analysis of A549. 1,primary Antibody was diluted at 1:200(4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 488 Secondary antibody was diluted at 1:1000(room temperature, 50min).3, Picture B: DAPI(blue) 10min. | Western Blot analysis of various cells using PUMA Polyclonal Antibody | Western blot analysis of lysate from 293 cells treated with EGF, using PUMA antibody. |
Key Facts
Product type
Primary Antibody›
Target: PUMA
| Target | PUMA |
| Also known as | BBC3; PUMA; Bcl-2-binding component 3; JFY-1; p53 up-regulated modulator of apoptosis |
| Concentration | 1 mg/ml |
| Working concentration | WB 1:500-2000 IF 1:100-300 ELISA 1:5000-20000 Not yet tested in other applications. |
| Species reactivity | Human;Mouse;Rat |
| Observed band | 23kD |
| Cellular localization | Mitochondrion . Localized to the mitochondria in order to induce cytochrome c release. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Platinum drug resistance;>>p53 signaling pathway;>>Apoptosis;>>Apoptosis - multiple species;>>Hippo signaling pathway;>>Huntington disease;>>Measles;>>Pathways in cancer;>>Colorectal cancer |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBIFELISA |
| Dilution range | WB 1:500-2000 IF 1:100-300 ELISA 1:5000-20000 Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human PUMA. AA range:120-169 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Why researchers trust ABMIUM
2,000+
Researchers trust us
50,000+
Citations
ISO 9001
Certified Collaborators
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000 IF 1:100-300 ELISA 1:5000-20000 Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
ABMIUM Validated™
IF
| Species | Dilution | Notes |
|---|
ABMIUM Validated™
ELISA
| Species | Dilution | Notes |
|---|
Specifications
Target
PUMA
Also known as
BBC3; PUMA; Bcl-2-binding component 3; JFY-1; p53 up-regulated modulator of apoptosis
Concentration
1 mg/ml
Working concentration
WB 1:500-2000 IF 1:100-300 ELISA 1:5000-20000 Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human;Mouse;Rat
Tested applications
WBIFELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human PUMA. AA range:120-169
Observed band
23kD
Cellular localization
Mitochondrion . Localized to the mitochondria in order to induce cytochrome c release.
Dilution range
WB 1:500-2000 IF 1:100-300 ELISA 1:5000-20000 Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage & Stability
Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
Customer Reviews
Recently Viewed