PP2A-Aβ rabbit pAb
PP2A-Aβ rabbit pAb from ELK Biotechnology Read more ›
Product Description
PP2A-Aβ rabbit pAb is an ELK Biotechnology antibody for research workflows involving PP2A-Aβ.
Background
This gene encodes a constant regulatory subunit of protein phosphatase 2. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The constant regulatory subunit A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. This gene encodes a beta isoform of the constant regulatory subunit A. Mutations in this gene have been associated with some lung and colon cancers. Alternatively spliced transcript variants have been described. [provided by RefSeq, Apr 2010],
Additional antibody information
| Specificity | PP2A-Aβ Polyclonal Antibody detects endogenous levels of PP2A-Aβ protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using PP2A-Aβ Polyclonal Antibody | Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using PPP2R1B Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of lysates from HeLa and Jurkat cells, using PPP2R1B Antibody. The lane on the right is blocked with the synthesized peptide. | Western blot analysis of the lysates from Jurkat cells using PPP2R1B antibody. |
Key Facts
| Target | PP2A-Aβ |
| Also known as | PPP2R1B; Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A beta isoform; PP2A subunit A isoform PR65-beta; PP2A subunit A isoform R1-beta |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/500 - 1/2000.IHC-p:1:50-300 ELISA: 1/20000. Not yet tested in other applications. |
| Species reactivity | Human;Mouse;Rat |
| Observed band | 66kD |
| Cellular localization | membrane raft,extracellular exosome, |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>mRNA surveillance pathway;>>Sphingolipid signaling pathway;>>Oocyte meiosis;>>PI3K-Akt signaling pathway;>>AMPK signaling pathway;>>Adrenergic signaling in cardiomyocytes;>>TGF-beta signaling pathway;>>Hippo signaling pathway;>>Tight junction;>>Dopaminergic synapse;>>Long-term depression;>>Chagas disease;>>Hepatitis C;>>Human papillomavirus infection |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBIHCIFELISA |
| Dilution range | Western Blot: 1/500 - 1/2000.IHC-p:1:50-300 ELISA: 1/20000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human PPP2R1B. AA range:552-601 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000.IHC-p:1:50-300 ELISA: 1/20000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.