PP2A-Aβ rabbit pAb from ELK Biotechnology Read more ›
PP2A-Aβ rabbit pAb is a research-use antibody from ELK Biotechnology for studying PP2A-Aβ in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
This gene encodes a constant regulatory subunit of protein phosphatase 2. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The constant regulatory subunit A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. This gene encodes a beta isoform of the constant regulatory subunit A. Mutations in this gene have been associated with some lung and colon cancers. Alternatively spliced transcript variants have been described. [provided by RefSeq, Apr 2010],
| Specificity | PP2A-Aβ Polyclonal Antibody detects endogenous levels of PP2A-Aβ protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using PP2A-Aβ Polyclonal Antibody | Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using PPP2R1B Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of lysates from HeLa and Jurkat cells, using PPP2R1B Antibody. The lane on the right is blocked with the synthesized peptide. | Western blot analysis of the lysates from Jurkat cells using PPP2R1B antibody. |
| Collaborator | ELK Biotechnology |
| Partner model | ES3247 |
| Target | PP2A-Aβ |
| Research areas | >>mRNA surveillance pathway, >>Sphingolipid signaling pathway, >>Oocyte meiosis, >>PI3K-Akt signaling pathway, >>AMPK signaling pathway, >>Adrenergic signaling in cardiomyocytes, >>TGF-beta signaling pathway, >>Hippo signaling pathway, >>Tight junction, >>Dopaminergic synapse, >>Long-term depression, >>Chagas disease, >>Hepatitis C, >>Human papillomavirus infection |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000.IHC-p:1:50-300 ELISA: 1/20000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human PPP2R1B. AA range:552-601 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Testis, |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000.IHC-p:1:50-300 ELISA: 1/20000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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