PI 3-kinase p85α rabbit pAb from ELK Biotechnology Read more ›
PI 3-kinase p85α rabbit pAb is a research-use antibody from ELK Biotechnology for studying PI3 Kinase P85α in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011],
| Specificity | PI 3-kinase p85α Polyclonal Antibody detects endogenous levels of PI 3-kinase p85α protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using PI 3-kinase p85α Polyclonal Antibody diluted at 1:1000 | Immunohistochemical analysis of paraffin-embedded Human placenta. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide. | Immunofluorescence analysis of HeLa cells, using PI3-kinase p85-alpha Antibody. The picture on the right is blocked with the synthesized peptide. | Immunohistochemistry analysis of paraffin-embedded human brain tissue, using PI3-kinase p85-alpha Antibody. The picture on the right is blocked with the synthesized peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES3199 |
| Target | PI3 Kinase P85α |
| Research areas | >>EGFR tyrosine kinase inhibitor resistance, >>Endocrine resistance, >>Platinum drug resistance, >>ErbB signaling pathway, >>Ras signaling pathway, >>Rap1 signaling pathway, >>cAMP signaling pathway, >>Chemokine signaling pathway, >>HIF-1 signaling pathway, >>FoxO signaling pathway, >>Phosphatidylinositol signaling system, >>Sphingolipid signaling pathway, >>Phospholipase D signaling pathway, >>Autophagy - animal, >>mTOR signaling pathway, >>PI3K-Akt signaling pathway, >>AMPK signaling pathway, >>Apoptosis, >>Longevity regulating pathway, >>Longevity regulating pathway - multiple species, >>Cellular senescence, >>Axon guidance, >>VEGF signaling pathway, >>Osteoclast differentiation, >>Focal adhesion, >>Signaling pathways regulating pluripotency of stem cells, >>Platelet activation, >>Neutrophil extracellular trap formation, >>Toll-like receptor signaling pathway, >>C-type lectin receptor signaling pathway, >>JAK-STAT signaling pathway, >>Natural killer cell mediated cytotoxicity, >>T cell receptor signaling pathway, > |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human PI3-kinase p85-alpha. AA range:573-622 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Isoform 2 is expressed in skeletal muscle and brain, and at lower levels in kidney and cardiac muscle. Isoform 2 and isoform 4 are present in skeletal muscle (at protein level). |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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