Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM97553

Peroxin 11β rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

Peroxin 11β rabbit pAb from ELK Biotechnology Read more ›

Peroxin 11β rabbit pAb
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Peroxin 11β rabbit pAb

Product Description

Peroxin 11β rabbit pAb is a research-use antibody from ELK Biotechnology for studying Peroxin 11β in WB, IHC, ELISA workflows with listed reactivity for Human, Mouse, Rat, Monkey. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

The protein encoded by this gene facilitates peroxisomal proliferation and interacts with PEX19. The encoded protein is found in the peroxisomal membrane. Several transcript variants, some protein-coding and some not protein-coding, have been found for this gene. [provided by RefSeq, Dec 2012],

Additional antibody information

Specificity Peroxin 11β Polyclonal Antibody detects endogenous levels of Peroxin 11β protein.
Validation evidence captions Western Blot analysis of various cells using Peroxin 11β Polyclonal Antibody diluted at 1:500 | Western blot analysis of lysates from COLO, HUVEC, COS7, and Jurkat cells, using PEX11B Antibody. The lane on the right is blocked with the synthesized peptide. | Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).

Key Facts

Product typePrimary Antibody Target: Peroxin 11β
Collaborator ELK Biotechnology
Partner model ES3177
Target Peroxin 11β
Research areas>>Peroxisome
Reactivity Human,Mouse,Rat,Monkey
Applications WB,IHC,ELISA
ConjugationUnconjugated
Dilution rangeWB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen The antiserum was produced against synthesized peptide derived from human PEX11B. AA range:91-140
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Eye,Liver,
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

4 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
Monkey
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Mouse
Rat
Monkey
ABMIUM Validated™ IHC
Species Dilution Notes
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
Peroxin 11β
Also known as
PEX11B; Peroxisomal membrane protein 11B; Peroxin-11B; Peroxisomal biogenesis factor 11B; Protein PEX11 homolog beta; PEX11-beta
Concentration
1 mg/ml
Working concentration
WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat,Monkey
Tested applications
WBIHCELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human PEX11B. AA range:91-140
Observed band
28kD
Cellular localization
Peroxisome membrane ; Single-pass membrane protein .
Dilution range
WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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