PARD3A rabbit pAb from ELK Biotechnology Read more ›
PARD3A rabbit pAb is a research-use antibody from ELK Biotechnology for studying PARD3A in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
This gene encodes a member of the PARD protein family. PARD family members interact with other PARD family members and other proteins; they affect asymmetrical cell division and direct polarized cell growth. Multiple alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Oct 2011],
| Specificity | PARD3A Polyclonal Antibody detects endogenous levels of PARD3A protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using PARD3A Polyclonal Antibody | Immunofluorescence analysis of HepG2 cells, using PARD3 Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of lysates from COLO205 cells, using PARD3 Antibody. The lane on the right is blocked with the synthesized peptide. | Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min). |
| Collaborator | ELK Biotechnology |
| Partner model | ES3149 |
| Target | PARD3A |
| Research areas | >>Rap1 signaling pathway, >>Chemokine signaling pathway, >>Neuroactive ligand-receptor interaction, >>Endocytosis, >>Axon guidance, >>Hippo signaling pathway, >>Adherens junction, >>Tight junction, >>Human papillomavirus infection |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human PARD3. AA range:1141-1190 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Widely expressed. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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