Olfactory receptor 52D1 rabbit pAb
Olfactory receptor 52D1 rabbit pAb from ELK Biotechnology Read more ›
Product Description
Olfactory receptor 52D1 rabbit pAb is an ELK Biotechnology antibody for research workflows involving Olfactory receptor 52D1.
Background
Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008],
Additional antibody information
| Specificity | Olfactory receptor 52D1 Polyclonal Antibody detects endogenous levels of Olfactory receptor 52D1 protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using Olfactory receptor 52D1 Polyclonal Antibody | Immunofluorescence analysis of MCF7 cells, using OR52D1 Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of lysates from COLO and COS7 cells, using OR52D1 Antibody. The lane on the right is blocked with the synthesized peptide. | Western blot analysis of the lysates from HepG2 cells using OR52D1 antibody. |
Key Facts
| Target | Olfactory receptor 52D1 |
| Also known as | OR52D1; Olfactory receptor 52D1; Odorant receptor HOR5'beta14; Olfactory receptor OR11-43 |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. |
| Species reactivity | Human;Monkey |
| Observed band | 35kD |
| Cellular localization | Cell membrane; Multi-pass membrane protein. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Olfactory transduction |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBIFELISA |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human OR52D1. AA range:269-318 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Reactivity & Application Validation
2 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Monkey |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. | — |
| Monkey | — | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.