Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM97506

Olfactory receptor 4K14 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

Olfactory receptor 4K14 rabbit pAb from ELK Biotechnology Read more ›

Olfactory receptor 4K14 rabbit pAb
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Olfactory receptor 4K14 rabbit pAb

Product Description

Olfactory receptor 4K14 rabbit pAb is a research-use antibody from ELK Biotechnology for studying Olfactory receptor 4K14 in WB, IF, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008],

Additional antibody information

Specificity Olfactory receptor 4K14 Polyclonal Antibody detects endogenous levels of Olfactory receptor 4K14 protein.
Validation evidence captions Western Blot analysis of various cells using Olfactory receptor 4K14 Polyclonal Antibody diluted at 1:2000 | Immunofluorescence analysis of A549 cells, using OR4K14 Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of lysates from HeLa, HepG2, and HUVEC cells, using OR4K14 Antibody. The lane on the right is blocked with the synthesized peptide. | Western blot analysis of the lysates from HepG2 cells using OR4K14 antibody.

Key Facts

Product typePrimary Antibody Target: Olfactory receptor 4K14
Collaborator ELK Biotechnology
Partner model ES3043
Target Olfactory receptor 4K14
Research areas>>Olfactory transduction
Reactivity Human,Rat,Mouse
Applications WB,IF,ELISA
ConjugationUnconjugated
Dilution rangeWestern Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen The antiserum was produced against synthesized peptide derived from human OR4K14. AA range:261-310
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
Rat
Mouse
ABMIUM Validated™ IF
Species Dilution Notes
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
Olfactory receptor 4K14
Also known as
OR4K14; Olfactory receptor 4K14; Olfactory receptor OR14-22
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Rat,Mouse
Tested applications
WBIFELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human OR4K14. AA range:261-310
Observed band
35kD
Cellular localization
Cell membrane; Multi-pass membrane protein.
Dilution range
Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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