Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM97472

OBCAM rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

OBCAM rabbit pAb from ELK Biotechnology Read more ›

OBCAM rabbit pAb
Zoom
OBCAM rabbit pAb

Product Description

OBCAM rabbit pAb is a research-use antibody from ELK Biotechnology for studying OBCAM in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat, Monkey. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene encodes a member of the IgLON subfamily in the immunoglobulin protein superfamily of proteins. The encoded preprotein is proteolytically processed to generate the mature protein. This protein is localized in the plasma membrane and may have an accessory role in opioid receptor function. This gene has an ortholog in rat and bovine. The opioid binding-cell adhesion molecule encoded by the rat gene binds opioid alkaloids in the presence of acidic lipids, exhibits selectivity for mu ligands and acts as a GPI-anchored protein. Since the encoded protein is highly conserved in species during evolution, it may have a fundamental role in mammalian systems. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Jan 2016],

Additional antibody information

Specificity OBCAM Polyclonal Antibody detects endogenous levels of OBCAM protein.
Validation evidence captions Western Blot analysis of various cells using OBCAM Polyclonal Antibody diluted at 1:1000 | Western blot analysis of lysate from COS7 cells, using OBCAM antibody.

Key Facts

Product typePrimary Antibody Target: OBCAM
Collaborator ELK Biotechnology
Partner model ES2996
Target OBCAM
Research areasAntibody Research
Reactivity Human,Mouse,Rat,Monkey
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWestern Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen The antiserum was produced against synthesized peptide derived from human OBCAM. AA range:152-201
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Amygdala,Brain,Fetal brain,Human amygdala,Occipital
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Why ABMIUM?
2,000+ Researchers trust us
50,000+ Citations
ISO 9001 Certified Collaborators
Independently handpicked Products selected for quality and fit

Application Validation Matrix

4 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
Monkey
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Mouse
Rat
Monkey
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
OBCAM
Also known as
OPCML; IGLON1; OBCAM; Opioid-binding protein/cell adhesion molecule; OBCAM; OPCML; Opioid-binding cell adhesion molecule; IgLON family member 1
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat,Monkey
Tested applications
WBELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human OBCAM. AA range:152-201
Observed band
58kD
Cellular localization
Cell membrane ; Lipid-anchor, GPI-anchor .
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

Customer Reviews

No customer reviews yet.