NRBF-2 rabbit pAb from ELK Biotechnology Read more ›
NRBF-2 rabbit pAb is a research-use antibody from ELK Biotechnology for studying NRBF-2 in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
function:May modulate transcriptional activation by target nuclear receptors. Can act as transcriptional activator (in vitro).,subunit:Interacts with PPARA, PPARD and PPARG. Interacts with RARA, RARG and RXRA in the presence of bound ligand.,tissue specificity:Detected in keratinocytes, liver and placenta.,
| Specificity | NRBF-2 Polyclonal Antibody detects endogenous levels of NRBF-2 protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using NRBF-2 Polyclonal Antibody | Western blot analysis of lysates from A549 cells, using NRBF2 Antibody. The lane on the right is blocked with the synthesized peptide. | Western blot analysis of the lysates from RAW264.7cells using NRBF2 antibody. |
| Collaborator | ELK Biotechnology |
| Partner model | ES2984 |
| Target | NRBF-2 |
| Research areas | >>Autophagy - animal, >>Alzheimer disease, >>Amyotrophic lateral sclerosis, >>Huntington disease, >>Spinocerebellar ataxia, >>Pathways of neurodegeneration - multiple diseases |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human NRBF2. AA range:140-189 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Detected in keratinocytes, liver and placenta (PubMed:15610520). Expressed in a subset of cells in pediatric medulloblastoma (PubMed:18619852). |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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