ABM97468
NRBF-2 rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
NRBF-2 rabbit pAb from ELK Biotechnology Read more ›
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Product Description
NRBF-2 rabbit pAb is an ELK Biotechnology antibody for research workflows involving NRBF-2.
Background
function:May modulate transcriptional activation by target nuclear receptors. Can act as transcriptional activator (in vitro).,subunit:Interacts with PPARA, PPARD and PPARG. Interacts with RARA, RARG and RXRA in the presence of bound ligand.,tissue specificity:Detected in keratinocytes, liver and placenta.,
Additional antibody information
| Specificity | NRBF-2 Polyclonal Antibody detects endogenous levels of NRBF-2 protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using NRBF-2 Polyclonal Antibody | Western blot analysis of lysates from A549 cells, using NRBF2 Antibody. The lane on the right is blocked with the synthesized peptide. | Western blot analysis of the lysates from RAW264.7cells using NRBF2 antibody. |
Key Facts
Product type
Primary Antibody›
Target: NRBF-2
| Target | NRBF-2 |
| Also known as | NRBF2; COPR; Nuclear receptor-binding factor 2; NRBF-2; Comodulator of PPAR and RXR |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. |
| Species reactivity | Human;Mouse;Rat |
| Observed band | 36kD |
| Cellular localization | Nucleus . Cytoplasm . Cytoplasmic vesicle . Cytoplasmic vesicle, autophagosome . |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Autophagy - animal;>>Alzheimer disease;>>Amyotrophic lateral sclerosis;>>Huntington disease;>>Spinocerebellar ataxia;>>Pathways of neurodegeneration - multiple diseases |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBELISA |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human NRBF2. AA range:140-189 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
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Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
ABMIUM Validated™
ELISA
| Species | Dilution | Notes |
|---|
Specifications
Target
NRBF-2
Also known as
NRBF2; COPR; Nuclear receptor-binding factor 2; NRBF-2; Comodulator of PPAR and RXR
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human;Mouse;Rat
Tested applications
WBELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human NRBF2. AA range:140-189
Observed band
36kD
Cellular localization
Nucleus . Cytoplasm . Cytoplasmic vesicle . Cytoplasmic vesicle, autophagosome .
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage & Stability
Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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