Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM97464

NPDC-1 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

NPDC-1 rabbit pAb from ELK Biotechnology Read more ›

NPDC-1 rabbit pAb
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NPDC-1 rabbit pAb

Product Description

NPDC-1 rabbit pAb is a research-use antibody from ELK Biotechnology for studying NPDC-1 in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

function:Suppresses oncogenic transformation in neural and non-neural cells and down-regulates neural cell proliferation. Might be involved in transcriptional regulation.,similarity:Belongs to the NPDC1/cab-1 family.,tissue specificity:Strongly expressed in adult brain; especially in hippocampus, frontal lobe and temporal lobe.,

Additional antibody information

Specificity NPDC-1 Polyclonal Antibody detects endogenous levels of NPDC-1 protein.
Validation evidence captions Western Blot analysis of various cells using NPDC-1 Polyclonal Antibody | Western blot analysis of lysates from HepG2 and LOVO cells, using NPDC1 Antibody. The lane on the right is blocked with the synthesized peptide. | Western blot analysis of the lysates from K562 cells using NPDC1 antibody.

Key Facts

Product typePrimary Antibody Target: NPDC-1
Collaborator ELK Biotechnology
Partner model ES2979
Target NPDC-1
Research areasAntibody Research
Reactivity Human,Mouse,Rat
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWestern Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen The antiserum was produced against synthesized peptide derived from human NPDC1. AA range:189-238
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Strongly expressed in adult brain; especially in hippocampus, frontal lobe and temporal lobe.
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Mouse
Rat
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
NPDC-1
Also known as
NPDC1; Neural proliferation differentiation and control protein 1; NPDC-1
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat
Tested applications
WBELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human NPDC1. AA range:189-238
Observed band
35kD
Cellular localization
Membrane ; Single-pass membrane protein .
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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