Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM97446

Nek5 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

Nek5 rabbit pAb from ELK Biotechnology Read more ›

Nek5 rabbit pAb 50µL/100µL
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Nek5 rabbit pAb 50µL/100µL

Product Description

Nek5 rabbit pAb is a research-use antibody from ELK Biotechnology for studying Nek5 in WB, ELISA workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,similarity:Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.,similarity:Contains 1 protein kinase domain.,

Additional antibody information

Specificity Nek5 Polyclonal Antibody detects endogenous levels of Nek5 protein.
Validation evidence captions Western Blot analysis of various cells using Nek5 Polyclonal Antibody | Western blot analysis of NEK5 Antibody. The lane on the right is blocked with the NEK5 peptide.

Key Facts

Product typePrimary Antibody Target: Nek5
Collaborator ELK Biotechnology
Partner model ES2918
Target Nek5
Research areasAntibody Research
Reactivity Human,Mouse
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWestern Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen The antiserum was produced against synthesized peptide derived from human NEK5. AA range:160-209
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Testis,
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

2 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.
Mouse
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
Nek5
Also known as
NEK5; Serine/threonine-protein kinase Nek5; Never in mitosis A-related kinase 5; NimA-related protein kinase 5
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse
Tested applications
WBELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human NEK5. AA range:160-209
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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