Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM97444

NEGR1 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

NEGR1 rabbit pAb from ELK Biotechnology Read more ›

NEGR1 rabbit pAb 50µL/100µL
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NEGR1 rabbit pAb 50µL/100µL

Product Description

NEGR1 rabbit pAb is a research-use antibody from ELK Biotechnology for studying NEGR1 in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

function:May be involved in cell-adhesion. May function as a trans-neural growth-promoting factor in regenerative axon sprouting in the mammalian brain.,similarity:Belongs to the immunoglobulin superfamily. IgLON family.,similarity:Contains 3 Ig-like C2-type (immunoglobulin-like) domains.,

Additional antibody information

Specificity NEGR1 Polyclonal Antibody detects endogenous levels of NEGR1 protein.
Validation evidence captions Western Blot analysis of various cells using NEGR1 Polyclonal Antibody | Western blot analysis of lysates from COLO cells, using NEGR1 Antibody. The lane on the right is blocked with the synthesized peptide. | Western blot analysis of the lysates from COLO205 cells using NEGR1 antibody.

Key Facts

Product typePrimary Antibody Target: NEGR1
Collaborator ELK Biotechnology
Partner model ES2915
Target NEGR1
Research areas>>Cell adhesion molecules
Reactivity Human,Mouse,Rat
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWestern Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen The antiserum was produced against synthesized peptide derived from human NEGR1. AA range:172-221
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Amygdala,Brain,Fetal kidney,Testis,
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Mouse
Rat
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
NEGR1
Also known as
NEGR1; IGLON4; Neuronal growth regulator 1; IgLON family member 4
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat
Tested applications
WBELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human NEGR1. AA range:172-221
Observed band
35kD
Cellular localization
Cell membrane ; Lipid-anchor, GPI-anchor .
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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