Myp rabbit pAb from ELK Biotechnology Read more ›
Myp rabbit pAb is a research-use antibody from ELK Biotechnology for studying Myp in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.
NOL3 encodes an anti-apoptotic protein nucleolar protein 3 that has been shown to down-regulate the enzyme activities of caspase 2, caspase 8 and tumor protein p53. Multiple transcript variants encoding different isoforms have been found for NOL3.
| Specificity | Myp Polyclonal Antibody detects endogenous levels of Myp protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using Myp Polyclonal Antibody | Immunohistochemistry analysis of paraffin-embedded human brain tissue, using ARC Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of lysates from HeLa cells, using ARC Antibody. The lane on the right is blocked with the synthesized peptide. | Western Blot analysis of lane1 mouse-brain, lane2 mouse-kidney, lane3 Hela. land4 MCF7, lane5 293T, lane6 mouse-muscle using primary antibody at 1:1000 dilution 4°C, overnight. Secondary antibody(catalog#:RS23920) was diluted at 1:10000 25°C，1.5hours |
| Collaborator | ELK Biotechnology |
| Partner model | ES2881 |
| Target | Myp |
| Research areas | Antibody Research |
| Reactivity | Human,Rat,Mouse |
| Applications | WB,IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human ARC. AA range:159-208 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Highly expressed in heart and skeletal muscle. Detected at low levels in placenta, liver, kidney and pancreas. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. | — |
| Rat | — | — |
| Mouse | — | — |
| Species | Dilution | Notes |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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