MST-3 rabbit pAb from ELK Biotechnology Read more ›
MST-3 rabbit pAb is a research-use antibody from ELK Biotechnology for studying MST3 in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
This gene encodes a serine/threonine protein kinase that functions upstream of mitogen-activated protein kinase (MAPK) signaling. The encoded protein is cleaved into two chains by caspases; the N-terminal fragment (MST3/N) translocates to the nucleus and promotes programmed cells death. There is a pseudogene for this gene on chromosome X. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2013],
| Specificity | MST-3 Polyclonal Antibody detects endogenous levels of MST-3 protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using MST-3 Polyclonal Antibody diluted at 1:1000 | Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using STK24 Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of lysates from COLO cells, using STK24 Antibody. The lane on the right is blocked with the synthesized peptide. | Western blot analysis of the lysates from COLO205 cells using STK24 antibody. |
| Collaborator | ELK Biotechnology |
| Partner model | ES2861 |
| Target | MST3 |
| Research areas | Antibody Research |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human STK24. AA range:319-368 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Isoform A is ubiquitous. Isoform B is expressed in brain with high expression in hippocampus and cerebral cortex. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
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| Species | Dilution | Notes |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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