Primary Antibody ISO 9001|ABMIUM Verified™
ABM97401

MRGF rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

MRGF rabbit pAb from ELK Biotechnology Read more ›

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MRGF rabbit pAb
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MRGF rabbit pAb

Product Description

MRGF rabbit pAb is an ELK Biotechnology antibody for research workflows involving MRGF.

Background

function:Orphan receptor. May bind to a neuropeptide and may regulate nociceptor function and/or development, including the sensation or modulation of pain.,similarity:Belongs to the G-protein coupled receptor 1 family.,similarity:Belongs to the G-protein coupled receptor 1 family. Mas subfamily.,

Additional antibody information

Specificity MRGF Polyclonal Antibody detects endogenous levels of MRGF protein.
Validation evidence captions Western Blot analysis of various cells using MRGF Polyclonal Antibody | Western blot analysis of lysates from HUVEC cells, using MRGRF Antibody. The lane on the right is blocked with the synthesized peptide. | Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).

Key Facts

Product type Primary Antibody Target: MRGF
Target MRGF
Also known as MRGPRF; GPR140; GPR168; MRGF; PSEC0142; Mas-related G-protein coupled receptor member F; Mas-related gene F protein; G-protein coupled receptor 140; G-protein coupled receptor 168
Concentration 1 mg/ml
Working concentration WB 1:500-2000;IHC-p 1:50-300
Species reactivity Human;Mouse;Rat
Observed band 40kD
Cellular localization Cell membrane; Multi-pass membrane protein.
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Research area Antibody Research
Host species Rabbit
Clonality Polyclonal
Isotype IgG
Conjugation Unconjugated
Tested applications
WBIHC
Dilution range WB 1:500-2000;IHC-p 1:50-300
Immunogen The antiserum was produced against synthesized peptide derived from human MRGRF. AA range:271-320
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Reactivity & Application Validation

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMCHIP
Human
Mouse
Rat
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:500-2000;IHC-p 1:50-300
Mouse
Rat
ABMIUM Validated™ IHC
Species Dilution Notes

Specifications

Target
MRGF
Also known as
MRGPRF; GPR140; GPR168; MRGF; PSEC0142; Mas-related G-protein coupled receptor member F; Mas-related gene F protein; G-protein coupled receptor 140; G-protein coupled receptor 168
Concentration
1 mg/ml
Working concentration
WB 1:500-2000;IHC-p 1:50-300
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human;Mouse;Rat
Tested applications
WBIHC
Immunogen
The antiserum was produced against synthesized peptide derived from human MRGRF. AA range:271-320
Observed band
40kD
Cellular localization
Cell membrane; Multi-pass membrane protein.
Dilution range
WB 1:500-2000;IHC-p 1:50-300
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ISO 9001
ISO 9001:2015 Certified

Manufactured under ISO 9001:2015 quality management standards.

RUO
For Research Use Only

Not intended for diagnostic or therapeutic use.

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