Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM97397

Mos rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

Mos rabbit pAb from ELK Biotechnology Read more ›

Mos rabbit pAb
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Mos rabbit pAb

Product Description

Mos rabbit pAb is a research-use antibody from ELK Biotechnology for studying Mos in WB, IHC, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

MOS is a serine/threonine kinase that activates the MAP kinase cascade through direct phosphorylation of the MAP kinase activator MEK (MAP2K1; MIM 176872) (Prasad et al., 2008 [PubMed 18246541]).[supplied by OMIM, Jul 2009],

Additional antibody information

Specificity Mos Polyclonal Antibody detects endogenous levels of Mos protein.
Validation evidence captions Western Blot analysis of various cells using Mos Polyclonal Antibody | Western blot analysis of lysates from 293 cells, using MOS Antibody. The lane on the right is blocked with the synthesized peptide. | Immunohistochemical analysis of paraffin-embedded human uterus. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).

Key Facts

Product typePrimary Antibody Target: Mos
Collaborator ELK Biotechnology
Partner model ES2813
Target Mos
Research areas>>Oocyte meiosis, >>Regulation of actin cytoskeleton, >>Progesterone-mediated oocyte maturation
Reactivity Human,Rat,Mouse
Applications WB,IHC,ELISA
ConjugationUnconjugated
Dilution rangeWB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen The antiserum was produced against synthesized peptide derived from human MOS. AA range:61-110
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Expressed specifically in testis during spermatogenesis.
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Rat
Mouse
ABMIUM Validated™ IHC
Species Dilution Notes
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
Mos
Also known as
MOS; Proto-oncogene serine/threonine-protein kinase mos; Oocyte maturation factor mos; Proto-oncogene c-Mos
Concentration
1 mg/ml
Working concentration
WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Rat,Mouse
Tested applications
WBIHCELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human MOS. AA range:61-110
Observed band
40kD
Cellular localization
intracellular,
Dilution range
WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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