MAGE-C2 rabbit pAb from ELK Biotechnology Read more ›
MAGE-C2 rabbit pAb is a research-use antibody from ELK Biotechnology for studying MAGE-C2 in WB, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.
This gene is a member of the MAGEC gene family. It is not expressed in normal tissues, except for testis, and is expressed in tumors of various histological types. This gene and the other MAGEC genes are clustered on chromosome Xq26-q27. [provided by RefSeq, Oct 2009],
| Specificity | MAGE-C2 Polyclonal Antibody detects endogenous levels of MAGE-C2 protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using MAGE-C2 Polyclonal Antibody diluted at 1:1000 | Western Blot analysis of 293 cells using MAGE-C2 Polyclonal Antibody diluted at 1:1000 | Western blot analysis of mouse-brain HELA KB SH-SY5Y 293T 3T3 lysis using MAGE-C2 antibody. Antibody was diluted at 1:1000 | Western blot analysis of MAGEC2 Antibody. The lane on the right is blocked with the MAGEC2 peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES2741 |
| Target | MAGE-C2 |
| Research areas | Antibody Research |
| Reactivity | Human,Rat,Mouse |
| Applications | WB,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Synthesized peptide derived from MAGE-C2 . at AA range: 160-240 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Not expressed in normal tissues, except in germ cells in the seminiferous tubules and in Purkinje cells of the cerebellum. Expressed in various tumors, including melanoma, lymphoma, as well as pancreatic cancer, mammary gland cancer, non-small cell lung cancer and liver cancer. In hepatocellular carcinoma, there is an inverse correlation between tumor differentiation and protein expression, i.e. the lower the differentiation, the higher percentage of expression. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications. | — |
| Rat | — | — |
| Mouse | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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