Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM97367

LTβR rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

LTβR rabbit pAb from ELK Biotechnology Read more ›

LTβR rabbit pAb
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LTβR rabbit pAb

Product Description

LTβR rabbit pAb is a research-use antibody from ELK Biotechnology for studying LTβR in WB, IF, ELISA workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

lymphotoxin beta receptor(LTBR) Homo sapiens This gene encodes a member of the tumor necrosis factor receptor superfamily. The major ligands of this receptor include lymphotoxin alpha/beta and tumor necrosis factor ligand superfamily member 14. The encoded protein plays a role in signalling during the development of lymphoid and other organs, lipid metabolism, immune response, and programmed cell death. Activity of this receptor has also been linked to carcinogenesis. Alternatively spliced transcript variants encoding multiple isoforms have been observed. [provided by RefSeq, Aug 2012],

Additional antibody information

Specificity LTβR Polyclonal Antibody detects endogenous levels of LTβR protein.
Validation evidence captions Western Blot analysis of various cells using LTβR Polyclonal Antibody | Immunofluorescence analysis of HeLa cells, using LTBR Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of lysates from HeLa and HepG2 cells, using LTBR Antibody. The lane on the right is blocked with the synthesized peptide.

Key Facts

Product typePrimary Antibody Target: LTβR
Collaborator ELK Biotechnology
Partner model ES2730
Target LTβR
Research areas>>Cytokine-cytokine receptor interaction, >>Viral protein interaction with cytokine and cytokine receptor, >>NF-kappa B signaling pathway, >>HIF-1 signaling pathway, >>Intestinal immune network for IgA production, >>Human T-cell leukemia virus 1 infection, >>Viral carcinogenesis
Reactivity Human,Mouse
Applications WB,IF,ELISA
ConjugationUnconjugated
Dilution rangeWestern Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen The antiserum was produced against synthesized peptide derived from human LTBR. AA range:11-60
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Liver,Lung,
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

2 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
Mouse
ABMIUM Validated™ IF
Species Dilution Notes
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
LTβR
Also known as
LTBR; D12S370; TNFCR; TNFR3; TNFRSF3; Tumor necrosis factor receptor superfamily member 3; Lymphotoxin-beta receptor; Tumor necrosis factor C receptor; Tumor necrosis factor receptor 2-related protein; Tumor necrosis factor receptor type II
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse
Tested applications
WBIFELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human LTBR. AA range:11-60
Observed band
50kD
Cellular localization
Membrane; Single-pass type I membrane protein.
Dilution range
Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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