IQGAP3 rabbit pAb from ELK Biotechnology Read more ›
IQGAP3 rabbit pAb is a research-use antibody from ELK Biotechnology for studying IQGAP3 in WB, IHC, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
similarity:Contains 1 CH (calponin-homology) domain.,similarity:Contains 1 Ras-GAP domain.,similarity:Contains 4 IQ domains.,
| Specificity | IQGAP3 Polyclonal Antibody detects endogenous levels of IQGAP3 protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using IQGAP3 Polyclonal Antibody diluted at 1:1000 | Western blot analysis of the lysates from 293 cells using IQGAP3 antibody. | Immunohistochemical analysis of paraffin-embedded human Gastric adenocarcinoma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min). |
| Collaborator | ELK Biotechnology |
| Partner model | ES2640 |
| Target | IQGAP3 |
| Research areas | >>Regulation of actin cytoskeleton |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,ELISA |
| Conjugation | Unconjugated |
| Dilution range | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human IQGAP3. AA range:107-156 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Embryonic kidney, |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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