ABM97325
IQGAP1 rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
IQGAP1 rabbit pAb from ELK Biotechnology Read more ›
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Product Description
IQGAP1 rabbit pAb is an ELK Biotechnology antibody for research workflows involving IQGAP1.
Background
IQ motif containing GTPase activating protein 1(IQGAP1) Homo sapiens This gene encodes a member of the IQGAP family. The protein contains four IQ domains, one calponin homology domain, one Ras-GAP domain and one WW domain. It interacts with components of the cytoskeleton, with cell adhesion molecules, and with several signaling molecules to regulate cell morphology and motility. Expression of the protein is upregulated by gene amplification in two gastric cancer cell lines. [provided by RefSeq, Jul 2008],
Additional antibody information
| Specificity | IQGAP1 Polyclonal Antibody detects endogenous levels of IQGAP1 protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using IQGAP1 Polyclonal Antibody diluted at 1:1000 | Western Blot analysis of HeLa cells using IQGAP1 Polyclonal Antibody diluted at 1:1000 | Western blot analysis of the lysates from K562 cells using IQGAP1 antibody. | Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min). |
Key Facts
Product type
Primary Antibody›
Target: IQGAP1
| Target | IQGAP1 |
| Also known as | IQGAP1; KIAA0051; Ras GTPase-activating-like protein IQGAP1; p195 |
| Concentration | 1 mg/ml |
| Working concentration | WB 1:500-2000;IHC-p 1:50-300 |
| Species reactivity | Human;Mouse |
| Observed band | 190kD |
| Cellular localization | Cell membrane . Nucleus . Cytoplasm . Subcellular distribution is regulated by the cell cycle, nuclear levels increase at G1/S phase (PubMed:20883816). . |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Adherens junction;>>Regulation of actin cytoskeleton;>>Proteoglycans in cancer |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBIHC |
| Dilution range | WB 1:500-2000;IHC-p 1:50-300 |
| Immunogen | The antiserum was produced against synthesized peptide derived from human IQGAP1. AA range:247-296 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
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Reactivity & Application Validation
2 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000;IHC-p 1:50-300 | — |
| Mouse | — | — |
ABMIUM Validated™
IHC
| Species | Dilution | Notes |
|---|
Specifications
Target
IQGAP1
Also known as
IQGAP1; KIAA0051; Ras GTPase-activating-like protein IQGAP1; p195
Concentration
1 mg/ml
Working concentration
WB 1:500-2000;IHC-p 1:50-300
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human;Mouse
Tested applications
WBIHC
Immunogen
The antiserum was produced against synthesized peptide derived from human IQGAP1. AA range:247-296
Observed band
190kD
Cellular localization
Cell membrane . Nucleus . Cytoplasm . Subcellular distribution is regulated by the cell cycle, nuclear levels increase at G1/S phase (PubMed:20883816). .
Dilution range
WB 1:500-2000;IHC-p 1:50-300
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage & Stability
Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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