Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM97305

IFITM3 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

IFITM3 rabbit pAb from ELK Biotechnology Read more ›

IFITM3 rabbit pAb
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IFITM3 rabbit pAb

Product Description

IFITM3 rabbit pAb is a research-use antibody from ELK Biotechnology for studying IFITM3 in WB, IF, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

The protein encoded by this gene is an interferon-induced membrane protein that helps confer immunity to influenza A H1N1 virus, West Nile virus, and dengue virus. Two transcript variants, only one of them protein-coding, have been found for this gene. Another variant encoding an N-terminally truncated isoform has been reported, but the full-length nature of this variant has not been determined. [provided by RefSeq, May 2012],

Additional antibody information

Specificity IFITM3 Polyclonal Antibody detects endogenous levels of IFITM3 protein.
Validation evidence captions Western Blot analysis of various cells using IFITM3 Polyclonal Antibody diluted at 1:2000 | Western Blot analysis of HeLa cells using IFITM3 Polyclonal Antibody diluted at 1:2000 | Immunofluorescence analysis of MCF7 cells, using IFM3 Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of lysates from HepG2 and HeLa cells, using IFM3 Antibody. The lane on the right is blocked with the synthesized peptide.

Key Facts

Product typePrimary Antibody Target: IFITM3
Collaborator ELK Biotechnology
Partner model ES2592
Target IFITM3
Research areasAntibody Research
Reactivity Human,Rat,Mouse
Applications WB,IF,ELISA
ConjugationUnconjugated
Dilution rangeWestern Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen The antiserum was produced against synthesized peptide derived from human IFM3. AA range:1-50
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Brain,Cervix,Vascular endothelial cell,
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
Rat
Mouse
ABMIUM Validated™ IF
Species Dilution Notes
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
IFITM3
Also known as
IFITM3; Interferon-induced transmembrane protein 3; Dispanin subfamily A member 2b; DSPA2b; Interferon-inducible protein 1-8U
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Rat,Mouse
Tested applications
WBIFELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human IFM3. AA range:1-50
Observed band
15kD
Cellular localization
Cell membrane ; Single-pass type II membrane protein . Late endosome membrane ; Single-pass type II membrane protein . Early endosome membrane ; Single-pass type II membrane protein. Lysosome membrane ; Single-pass type II membrane protein . Cytoplasm, perinuclear region . Co-localizes with BRI3 isoform 1 at the perinuclear region. .
Dilution range
Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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