ABM97300
HSP40 rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
HSP40 rabbit pAb from ELK Biotechnology Read more ›
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Product Description
HSP40 rabbit pAb is an ELK Biotechnology antibody for research workflows involving HSP40.
Background
This gene encodes a member of the DnaJ or Hsp40 (heat shock protein 40 kD) family of proteins. DNAJ family members are characterized by a highly conserved amino acid stretch called the 'J-domain' and function as one of the two major classes of molecular chaperones involved in a wide range of cellular events, such as protein folding and oligomeric protein complex assembly. The encoded protein is a molecular chaperone that stimulates the ATPase activity of Hsp70 heat-shock proteins in order to promote protein folding and prevent misfolded protein aggregation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015],
Additional antibody information
| Specificity | HSP40 Polyclonal Antibody detects endogenous levels of HSP40 protein. |
|---|---|
| Validation evidence captions | Immunofluorescence analysis of Hela cell. 1,HSP40 Polyclonal Antibody(red) was diluted at 1:200(4° overnight). Caspase-8 Monoclonal Antibody(2G12)(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog:RS3611 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog:RS3208 was diluted at 1:1000(room temperature, 50min). | Western Blot analysis of various cells using HSP40 Polyclonal Antibody | Immunofluorescence analysis of NIH/3T3 cells, using HSP40 Antibody. The picture on the right is blocked with the synthesized peptide. | Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using HSP40 Antibody. The picture on the right is blocked with the synthesized peptide. |
Key Facts
Product type
Primary Antibody›
Target: HSP40
| Target | HSP40 |
| Also known as | DNAJB1; DNAJ1; HDJ1; HSPF1; DnaJ homolog subfamily B member 1; DnaJ protein homolog 1; Heat shock 40 kDa protein 1; HSP40; Heat shock protein 40; Human DnaJ protein 1; hDj-1 |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Species reactivity | Human;Mouse;Rat |
| Observed band | 45kD |
| Cellular localization | Cytoplasm . Nucleus . Nucleus, nucleolus . Translocates rapidly from the cytoplasm to the nucleus, and especially to the nucleoli, upon heat shock. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Protein processing in endoplasmic reticulum;>>Influenza A |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBIHCIFELISA |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human HSP40. AA range:271-320 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
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Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
ABMIUM Validated™
IHC
| Species | Dilution | Notes |
|---|
ABMIUM Validated™
IF
| Species | Dilution | Notes |
|---|
ABMIUM Validated™
ELISA
| Species | Dilution | Notes |
|---|
Specifications
Target
HSP40
Also known as
DNAJB1; DNAJ1; HDJ1; HSPF1; DnaJ homolog subfamily B member 1; DnaJ protein homolog 1; Heat shock 40 kDa protein 1; HSP40; Heat shock protein 40; Human DnaJ protein 1; hDj-1
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human;Mouse;Rat
Tested applications
WBIHCIFELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human HSP40. AA range:271-320
Observed band
45kD
Cellular localization
Cytoplasm . Nucleus . Nucleus, nucleolus . Translocates rapidly from the cytoplasm to the nucleus, and especially to the nucleoli, upon heat shock.
Dilution range
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage & Stability
Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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