Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM97249

GPR83 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

GPR83 rabbit pAb from ELK Biotechnology Read more ›

GPR83 rabbit pAb
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GPR83 rabbit pAb

Product Description

GPR83 rabbit pAb is a research-use antibody from ELK Biotechnology for studying GPR83 in WB, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

function:Orphan receptor. Could be a neuropeptide y receptor.,similarity:Belongs to the G-protein coupled receptor 1 family.,tissue specificity:Brain specific.,

Additional antibody information

Specificity GPR83 Polyclonal Antibody detects endogenous levels of GPR83 protein.
Validation evidence captions Western Blot analysis of various cells using GPR83 Polyclonal Antibody | Western blot analysis of GPR83 Antibody. The lane on the right is blocked with the GPR83 peptide. | Western blot analysis of the lysates from HeLa cells using GPR83 antibody.

Key Facts

Product typePrimary Antibody Target: GPR83
Collaborator ELK Biotechnology
Partner model ES2468
Target GPR83
Research areas>>Neuroactive ligand-receptor interaction
Reactivity Human,Rat,Mouse
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWestern Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen The antiserum was produced against synthesized peptide derived from human GPR83. AA range:361-410
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Highly expressed in the brain and spinal cord, and found in lower concentrations in the thymus and other tissues.
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.
Rat
Mouse
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
GPR83
Also known as
GPR83; GPR72; KIAA1540; Probable G-protein coupled receptor 83; G-protein coupled receptor 72
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Rat,Mouse
Tested applications
WBELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human GPR83. AA range:361-410
Observed band
48kD
Cellular localization
Cell membrane ; Multi-pass membrane protein . Colocalizes with GPR171 in the paraventricular nucleus. Colocalizes with the ghrelin receptor GHSR1A in the hypothalamus. .
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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