GM2/GD2 synthase rabbit pAb from ELK Biotechnology Read more ›
GM2/GD2 synthase rabbit pAb is a research-use antibody from ELK Biotechnology for studying GM2/GD2 synthase in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
beta-1,4-N-acetyl-galactosaminyltransferase 1(B4GALNT1) Homo sapiens GM2 and GD2 gangliosides are sialic acid-containing glycosphingolipids. GalNAc-T is the enzyme involved in the biosynthesis of G(M2) and G(D2) glycosphingolipids. GalNAc-T catalyzes the transfer of GalNAc into G(M3) and G(D3) by a beta-1,4 linkage, resulting in the synthesis of G(M2) and G(D2), respectively. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2013],
| Specificity | GM2/GD2 synthase Polyclonal Antibody detects endogenous levels of GM2/GD2 synthase protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using GM2/GD2 synthase Polyclonal Antibody | Western blot analysis of the lysates from COLO205 cells using B4GALNT1 antibody. |
| Collaborator | ELK Biotechnology |
| Partner model | ES2440 |
| Target | GM2/GD2 synthase |
| Research areas | >>Sphingolipid metabolism, >>Glycosphingolipid biosynthesis - ganglio series, >>Metabolic pathways |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human B4GALNT1. AA range:281-330 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Brain, |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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