Glucosidase IIβ rabbit pAb from ELK Biotechnology Read more ›
Glucosidase IIβ rabbit pAb is a research-use antibody from ELK Biotechnology for studying Glucosidase IIβ in WB, IF, ELISA workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.
This gene encodes the beta-subunit of glucosidase II, an N-linked glycan-processing enzyme in the endoplasmic reticulum. The encoded protein is an acidic phosphoprotein known to be a substrate for protein kinase C. Mutations in this gene have been associated with the autosomal dominant polycystic liver disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014],
| Specificity | Glucosidase IIβ Polyclonal Antibody detects endogenous levels of Glucosidase IIβ protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using Glucosidase IIβ Polyclonal Antibody | Western Blot analysis of A549 cells using Glucosidase IIβ Polyclonal Antibody | Immunofluorescence analysis of HeLa cells, using GLU2B Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of lysates from HepG2, 293, and HUVEC cells, using GLU2B Antibody. The lane on the right is blocked with the synthesized peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES2434 |
| Target | Glucosidase IIβ |
| Research areas | >>Protein processing in endoplasmic reticulum |
| Reactivity | Human,Mouse |
| Applications | WB,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human GLU2B. AA range:81-130 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Lung,Lymphocyte,Platelet, |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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