GCSc-γ rabbit pAb from ELK Biotechnology Read more ›
GCSc-γ rabbit pAb is a research-use antibody from ELK Biotechnology for studying GCSc-γ in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.[provided by RefSeq, Oct 2010],
| Specificity | GCSc-γ Polyclonal Antibody detects endogenous levels of GCSc-γ protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using GCSc-γ Polyclonal Antibody | Western blot analysis of lysate from A549 cells, using GCSc-γ antibody. |
| Collaborator | ELK Biotechnology |
| Partner model | ES2414 |
| Target | GCSc-γ |
| Research areas | >>Cysteine and methionine metabolism, >>Glutathione metabolism, >>Metabolic pathways, >>Biosynthesis of cofactors, >>Ferroptosis |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human GCSc-gamma. AA range:266-315 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Foreskin fibroblast,Hippocampus,Liver,Testis,Thalamus, |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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