GADD 45γ rabbit pAb from ELK Biotechnology Read more ›
GADD 45γ rabbit pAb is a research-use antibody from ELK Biotechnology for studying GADD 45γ in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The protein encoded by this gene responds to environmental stresses by mediating activation of the p38/JNK pathway via MTK1/MEKK4 kinase. The GADD45G is highly expressed in placenta. [provided by RefSeq, Jul 2008],
| Specificity | GADD 45γ Polyclonal Antibody detects endogenous levels of GADD 45γ protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using GADD 45γ Polyclonal Antibody | Western blot analysis of GA45G Antibody. The lane on the right is blocked with the GA45G peptide. | Immunofluorescence analysis of A549 cells, using GA45G Antibody. The picture on the right is blocked with the synthesized peptide. | Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using GA45G Antibody. The picture on the right is blocked with the synthesized peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES2391 |
| Target | GADD 45γ |
| Research areas | >>MAPK signaling pathway, >>NF-kappa B signaling pathway, >>FoxO signaling pathway, >>Cell cycle, >>p53 signaling pathway, >>Apoptosis, >>Cellular senescence, >>Epstein-Barr virus infection, >>Pathways in cancer, >>Transcriptional misregulation in cancer, >>Colorectal cancer, >>Pancreatic cancer, >>Endometrial cancer, >>Glioma, >>Thyroid cancer, >>Basal cell carcinoma, >>Melanoma, >>Chronic myeloid leukemia, >>Small cell lung cancer, >>Non-small cell lung cancer, >>Breast cancer, >>Hepatocellular carcinoma, >>Gastric cancer |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human GA45G. AA range:101-150 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Brain,Colon carcinoma,Lung,Placenta, |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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