GABAA Rα4 rabbit pAb from ELK Biotechnology Read more ›
GABAA Rα4 rabbit pAb is a research-use antibody from ELK Biotechnology for studying GABA A Receptor α4 in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
Gamma-aminobutyric acid (GABA) is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. At least 16 distinct subunits of GABA-A receptors have been identified. This gene encodes subunit alpha-4, which is involved in the etiology of autism and eventually increases autism risk through interaction with another subunit, gamma-aminobutyric acid receptor beta-1 (GABRB1). Alternatively spliced transcript variants encoding different isoforms have been found in this gene.[provided by RefSeq, Feb 2011],
| Specificity | GABAA Rα4 Polyclonal Antibody detects endogenous levels of GABAA Rα4 protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using GABAA Rα4 Polyclonal Antibody diluted at 1:1000 | Western blot analysis of GABRA4 Antibody. The lane on the right is blocked with the GABRA4 peptide. | Western blot analysis of the lysates from COLO205 cells using GABRA4 antibody. |
| Collaborator | ELK Biotechnology |
| Partner model | ES2387 |
| Target | GABA A Receptor α4 |
| Research areas | >>Neuroactive ligand-receptor interaction, >>Retrograde endocannabinoid signaling, >>GABAergic synapse, >>Taste transduction, >>Morphine addiction, >>Nicotine addiction |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human GABRA4. AA range:81-130 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Brain,Brain cortex, |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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