GABAA Rα4 rabbit pAb
GABAA Rα4 rabbit pAb from ELK Biotechnology Read more ›
Product Description
GABAA Rα4 rabbit pAb is an ELK Biotechnology antibody for research workflows involving GABA A Receptor α4.
Background
Gamma-aminobutyric acid (GABA) is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. At least 16 distinct subunits of GABA-A receptors have been identified. This gene encodes subunit alpha-4, which is involved in the etiology of autism and eventually increases autism risk through interaction with another subunit, gamma-aminobutyric acid receptor beta-1 (GABRB1). Alternatively spliced transcript variants encoding different isoforms have been found in this gene.[provided by RefSeq, Feb 2011],
Additional antibody information
| Specificity | GABAA Rα4 Polyclonal Antibody detects endogenous levels of GABAA Rα4 protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using GABAA Rα4 Polyclonal Antibody diluted at 1:1000 | Western blot analysis of GABRA4 Antibody. The lane on the right is blocked with the GABRA4 peptide. | Western blot analysis of the lysates from COLO205 cells using GABRA4 antibody. |
Key Facts
| Target | GABA A Receptor α4 |
| Also known as | GABRA4; Gamma-aminobutyric acid receptor subunit alpha-4; GABA(A) receptor subunit alpha-4 |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. |
| Species reactivity | Human;Mouse;Rat |
| Observed band | 60kD |
| Cellular localization | Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Neuroactive ligand-receptor interaction;>>Retrograde endocannabinoid signaling;>>GABAergic synapse;>>Taste transduction;>>Morphine addiction;>>Nicotine addiction |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBELISA |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human GABRA4. AA range:81-130 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
|---|
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.