FRS2 rabbit pAb from ELK Biotechnology Read more ›
FRS2 rabbit pAb is a research-use antibody from ELK Biotechnology for studying FRS2 in WB, ELISA workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.
function:Adapter protein that links FGR and NGF receptors to downstream signaling pathways. Involved in the activation of MAP kinases. Modulates signaling via SHC1 by competing for a common binding site on NTRK1.,PTM:Phosphorylated on tyrosine residues upon stimulation by NGF.,PTM:Ubiquitinated when tyrosine phosphorylated and in a complex with GRB2. The unphosphorylated form is not subject to ubiquitination.,sequence caution:Translated as stop.,similarity:Contains 1 IRS-type PTB domain.,subcellular location:Cytoplasmic, membrane-bound.,subunit:Part of a complex containing FRS2, GRB2 and SOS1. Part of a complex containing GRB2 and CBL. Binds RET (By similarity). Binds FGFR1, SUC1, NTRK1, NTRK2, NTRK3 and SRC. The tyrosine-phosphorylated protein binds the SH2 domains of GRB2 and PTPN11.,tissue specificity:Highly expressed in heart, brain, spleen, lung, liver, skeletal muscle, kidney and testis.,
| Specificity | FRS2 Polyclonal Antibody detects endogenous levels of FRS2 protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using FRS2 Polyclonal Antibody diluted at 1:1000 | Western Blot analysis of HepG2 cells using FRS2 Polyclonal Antibody diluted at 1:1000 | Western blot analysis of KB lysis using FRS2 antibody. Antibody was diluted at 1:1000 | Western blot analysis of lysates from HepG2 cells, using FRS2 Antibody. The lane on the right is blocked with the synthesized peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES2376 |
| Target | FRS2 |
| Research areas | >>Thermogenesis, >>Neurotrophin signaling pathway, >>Proteoglycans in cancer |
| Reactivity | Human,Mouse |
| Applications | WB,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human FRS2. AA range:162-211 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Highly expressed in heart, brain, spleen, lung, liver, skeletal muscle, kidney and testis. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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