Flotillin-2 rabbit pAb from ELK Biotechnology Read more ›
Flotillin-2 rabbit pAb is a research-use antibody from ELK Biotechnology for studying Flotillin-2 in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
Caveolae are small domains on the inner cell membrane involved in vesicular trafficking and signal transduction. This gene encodes a caveolae-associated, integral membrane protein, which is thought to function in neuronal signaling. [provided by RefSeq, Jul 2008],
| Specificity | Flotillin-2 Polyclonal Antibody detects endogenous levels of Flotillin-2 protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using Flotillin-2 Polyclonal Antibody | Western blot analysis of lysate from A549 cells, using Flotillin-2 antibody. | Western blot analysis of 1) A431, 2)Mouse Brain Tissue, 3) Rat Brain Tissue with Flotllin-2 Rabbit pAb diluted at 1:2,000. | Immunohistochemical analysis of paraffin-embedded Human Lung Carcinoma using Flotillin-2 Rabbit pAb diluted at 1:200 |
| Collaborator | ELK Biotechnology |
| Partner model | ES2353 |
| Target | Flotillin-2 |
| Research areas | >>Insulin signaling pathway |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Flotillin-2. AA range:95-144 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | In skin, expressed in epidermis and epidermal appendages but not in dermis. Expressed in all layers of the epidermis except the basal layer. In hair follicles, expressed in the suprabasal layer but not the basal layer. Also expressed in melanoma and carcinoma cell lines, fibroblasts and foreskin melanocytes. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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