FGF-22 rabbit pAb from ELK Biotechnology Read more ›
FGF-22 rabbit pAb is a research-use antibody from ELK Biotechnology for studying FGF-22 in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. The mouse homolog of this gene was found to be preferentially expressed in the inner root sheath of the hair follicle, which suggested a role in hair development. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014],
| Specificity | FGF-22 Polyclonal Antibody detects endogenous levels of FGF-22 protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using FGF-22 Polyclonal Antibody diluted at 1:1000 | Immunofluorescence analysis of MCF7 cells, using FGF22 Antibody. The picture on the right is blocked with the synthesized peptide. | Immunohistochemistry analysis of paraffin-embedded human ovary tissue, using FGF22 Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of lysates from Jurkat and HT-29 cells, using FGF22 Antibody. The lane on the right is blocked with the synthesized peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES2340 |
| Target | FGF-22 |
| Research areas | >>MAPK signaling pathway, >>Ras signaling pathway, >>Rap1 signaling pathway, >>Calcium signaling pathway, >>PI3K-Akt signaling pathway, >>Regulation of actin cytoskeleton, >>Pathways in cancer, >>Chemical carcinogenesis - receptor activation, >>Melanoma, >>Breast cancer, >>Gastric cancer |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human FGF22. AA range:71-120 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Placenta, |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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