Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM97152

ELOVL4 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

ELOVL4 rabbit pAb from ELK Biotechnology Read more ›

ELOVL4 rabbit pAb
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ELOVL4 rabbit pAb

Product Description

ELOVL4 rabbit pAb is a research-use antibody from ELK Biotechnology for studying ELOVL4 in WB, ELISA workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene encodes a membrane-bound protein which is a member of the ELO family, proteins which participate in the biosynthesis of fatty acids. Consistent with the expression of the encoded protein in photoreceptor cells of the retina, mutations and small deletions in this gene are associated with Stargardt-like macular dystrophy (STGD3) and autosomal dominant Stargardt-like macular dystrophy (ADMD), also referred to as autosomal dominant atrophic macular degeneration. [provided by RefSeq, Jul 2008],

Additional antibody information

Specificity ELOVL4 Polyclonal Antibody detects endogenous levels of ELOVL4 protein.
Validation evidence captions Western Blot analysis of various cells using ELOVL4 Polyclonal Antibody diluted at 1:1000 | Western blot analysis of lysates from HeLa cells, using ELOVL4 Antibody. The lane on the right is blocked with the synthesized peptide.

Key Facts

Product typePrimary Antibody Target: ELOVL4
Collaborator ELK Biotechnology
Partner model ES2255
Target ELOVL4
Research areas>>Fatty acid elongation, >>Biosynthesis of unsaturated fatty acids, >>Metabolic pathways, >>Fatty acid metabolism
Reactivity Human,Mouse
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWestern Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen The antiserum was produced against synthesized peptide derived from human ELOVL4. AA range:41-90
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Expressed in the retina and at much lower level in the brain. Ubiquitous, highest expression in thymus, followed by testis, small intestine, ovary, and prostate. Little or no expression in heart, lung, liver, or leukocates.
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

2 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Mouse
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
ELOVL4
Also known as
ELOVL4; Elongation of very long chain fatty acids protein 4; 3-keto acyl-CoA synthase ELOVL4; ELOVL fatty acid elongase 4; ELOVL FA elongase 4
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse
Tested applications
WBELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human ELOVL4. AA range:41-90
Observed band
37kD
Cellular localization
Endoplasmic reticulum membrane ; Multi-pass membrane protein .
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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