ABM97149
eIF2Bγ rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
eIF2Bγ rabbit pAb from ELK Biotechnology Read more ›
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Product Description
eIF2Bγ rabbit pAb is an ELK Biotechnology antibody for research workflows involving eIF2Bγ.
Background
The protein encoded by this gene is one of the subunits of initiation factor eIF2B, which catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP. It has also been found to function as a cofactor of hepatitis C virus internal ribosome entry site-mediated translation. Mutations in this gene have been associated with leukodystrophy with vanishing white matter. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009],
Additional antibody information
| Specificity | eIF2Bγ Polyclonal Antibody detects endogenous levels of eIF2Bγ protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using eIF2Bγ Polyclonal Antibody diluted at 1:1000 | Western Blot analysis of K562 cells using eIF2Bγ Polyclonal Antibody diluted at 1:1000 |
Key Facts
Product type
Primary Antibody›
Target: eIF2Bγ
| Target | eIF2Bγ |
| Also known as | EIF2B3; Translation initiation factor eIF-2B subunit gamma; eIF-2B GDP-GTP exchange factor subunit gamma |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications. |
| Species reactivity | Human;Mouse |
| Observed band | 50kD |
| Cellular localization | cytoplasm,cytosol,eukaryotic translation initiation factor 2B complex, |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Herpes simplex virus 1 infection |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBELISA |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications. |
| Immunogen | Synthesized peptide derived from eIF2Bγ . at AA range: 240-320 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
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Reactivity & Application Validation
2 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications. | — |
| Mouse | — | — |
ABMIUM Validated™
ELISA
| Species | Dilution | Notes |
|---|
Specifications
Target
eIF2Bγ
Also known as
EIF2B3; Translation initiation factor eIF-2B subunit gamma; eIF-2B GDP-GTP exchange factor subunit gamma
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human;Mouse
Tested applications
WBELISA
Immunogen
Synthesized peptide derived from eIF2Bγ . at AA range: 240-320
Observed band
50kD
Cellular localization
cytoplasm,cytosol,eukaryotic translation initiation factor 2B complex,
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage & Stability
Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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