Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM97147

EGFL5 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

EGFL5 rabbit pAb from ELK Biotechnology Read more ›

EGFL5 rabbit pAb
Zoom
EGFL5 rabbit pAb

Product Description

EGFL5 rabbit pAb is a research-use antibody from ELK Biotechnology for studying EGFL5 in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

caution:It is uncertain whether Met-1 or Met-9 is the initiator.,polymorphism:The poly-Ala stretch (positions 24 to 31) may be polymorphic and varies from 6 to 8 Ala residues.,sequence caution:Intron retention.,similarity:Contains 5 laminin EGF-like domains.,

Additional antibody information

Specificity EGFL5 Polyclonal Antibody detects endogenous levels of EGFL5 protein.
Validation evidence captions Western Blot analysis of various cells using EGFL5 Polyclonal Antibody diluted at 1:500 | Western blot analysis of lysates from COLO cells, using MEGF9 Antibody. The lane on the right is blocked with the synthesized peptide.

Key Facts

Product typePrimary Antibody Target: EGFL5
Collaborator ELK Biotechnology
Partner model ES2233
Target EGFL5
Research areasAntibody Research
Reactivity Human,Mouse,Rat
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWestern Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen The antiserum was produced against synthesized peptide derived from human MEGF9. AA range:383-432
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Brain,
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Why ABMIUM?
2,000+ Researchers trust us
50,000+ Citations
ISO 9001 Certified Collaborators
Independently handpicked Products selected for quality and fit

Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Mouse
Rat
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
EGFL5
Also known as
MEGF9; EGFL5; KIAA0818; Multiple epidermal growth factor-like domains protein 9; Multiple EGF-like domains protein 9; Epidermal growth factor-like protein 5; EGF-like protein 5
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat
Tested applications
WBELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human MEGF9. AA range:383-432
Observed band
63kD
Cellular localization
Membrane ; Single-pass type I membrane protein .
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

Customer Reviews

No customer reviews yet.