ABM97127
DNA pol α rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
DNA pol α rabbit pAb from ELK Biotechnology Read more ›
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Product Description
DNA pol α rabbit pAb is an ELK Biotechnology antibody for research workflows involving DNA pol α.
Background
This gene encodes the catalytic subunit of DNA polymerase, which together with a regulatory and two primase subunits, forms the DNA polymerase alpha complex. The catalytic subunit plays an essential role in the initiation of DNA replication. [provided by RefSeq, Mar 2010],
Additional antibody information
| Specificity | DNA pol α Polyclonal Antibody detects endogenous levels of DNA pol α protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using DNA pol α Polyclonal Antibody diluted at 1:1000 | Western blot analysis of lysates from HepG2, 293, and Jurkat cells, using DNA Polymerase alpha Antibody. The lane on the right is blocked with the synthesized peptide. |
Key Facts
Product type
Primary Antibody›
Target: DNA pol α
| Target | DNA pol α |
| Also known as | POLA1; POLA; DNA polymerase alpha catalytic subunit; DNA polymerase alpha catalytic subunit p180 |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. |
| Species reactivity | Human;Rat;Mouse; |
| Observed band | 165kD |
| Cellular localization | Nucleus . Cytoplasm, cytosol . In the cytosol, colocalizes with RNA:DNA hybrids with a speckled pattern. . |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>DNA replication |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBELISA |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human DNA Polymerase alpha. AA range:81-130 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
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Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. | — |
| Rat | — | — |
| Mouse | — | — |
ABMIUM Validated™
ELISA
| Species | Dilution | Notes |
|---|
Specifications
Target
DNA pol α
Also known as
POLA1; POLA; DNA polymerase alpha catalytic subunit; DNA polymerase alpha catalytic subunit p180
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human;Rat;Mouse;
Tested applications
WBELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human DNA Polymerase alpha. AA range:81-130
Observed band
165kD
Cellular localization
Nucleus . Cytoplasm, cytosol . In the cytosol, colocalizes with RNA:DNA hybrids with a speckled pattern. .
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage & Stability
Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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