Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM97120

DHHC-7 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

DHHC-7 rabbit pAb from ELK Biotechnology Read more ›

DHHC-7 rabbit pAb
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DHHC-7 rabbit pAb

Product Description

DHHC-7 rabbit pAb is a research-use antibody from ELK Biotechnology for studying DHHC-7 in WB, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

catalytic activity:Palmitoyl-CoA + protein-cysteine = S-palmitoyl protein + CoA.,domain:The DHHC domain is required for palmitoyltransferase activity.,function:Palmitoyltransferase with broad specificity. Palmitoylates SNAP25 and DLG4/PSD95. May palmitoylate GABA receptors on their gamma subunit (GABRG1, GABRG2 and GABRG3) and regulate their synaptic clustering and/or cell surface stability.,similarity:Belongs to the DHHC palmitoyltransferase family.,similarity:Contains 1 DHHC-type zinc finger.,

Additional antibody information

Specificity DHHC-7 Polyclonal Antibody detects endogenous levels of DHHC-7 protein.
Validation evidence captions Western Blot analysis of various cells using DHHC-7 Polyclonal Antibody | Western blot analysis of lysates from HUVEC cells, using ZDHHC7 Antibody. The lane on the right is blocked with the synthesized peptide. | Western blot analysis of the lysates from HUVECcells using ZDHHC7 antibody.

Key Facts

Product typePrimary Antibody Target: DHHC-7
Collaborator ELK Biotechnology
Partner model ES2168
Target DHHC-7
Research areasAntibody Research
Reactivity Human,Rat,Mouse
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWestern Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen The antiserum was produced against synthesized peptide derived from human ZDHHC7. AA range:259-308
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Duodenal mucosa,Hepatoma,Skin,Teratocarcinoma,
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Rat
Mouse
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
DHHC-7
Also known as
ZDHHC7; ZNF370; Palmitoyltransferase ZDHHC7; Zinc finger DHHC domain-containing protein 7; DHHC-7; Zinc finger protein 370
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Rat,Mouse
Tested applications
WBELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human ZDHHC7. AA range:259-308
Observed band
35kD
Cellular localization
Golgi apparatus membrane ; Multi-pass membrane protein .
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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