ABM97082
Eif4b Rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
Eif4b Rabbit pAb from ELK Biotechnology Read more ›
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Product Description
Eif4b Rabbit pAb is an ELK Biotechnology antibody for research workflows involving eIF4B.
Background
cRequired for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.,similarity:Contains 1 RRM (RNA recognition motif) domain.,subunit:Self-associates and interacts with EIF3 p170 subunit.
Additional antibody information
| Specificity | This antibody detects endogenous levels of Eif4b at Human, Mouse,Rat |
|---|---|
| Validation evidence captions | Western blot analysis of 1)Hela Cell, 2) 3T3 Cell, 3)PC12 Cell Lysate using Eif4bRabbit Polyclonal Antibody diluted at 1:2,000. | Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min). |
Key Facts
Product type
Primary Antibody›
Target: eIF4B
| Target | eIF4B |
| Also known as | Eukaryotic translation initiation factor 4B (eIF-4B) |
| Concentration | 1 mg/ml |
| Working concentration | IHC-p1:50-200 ,WB 1:1000-2000 |
| Species reactivity | Human; Mouse;Rat |
| Observed band | 69kD |
| Cellular localization | cytosol,polysome,eukaryotic translation initiation factor 4F complex,dendrite,neuronal cell body, |
| Purity | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.77% sodium azide. |
| Research area | >>mTOR signaling pathway;>>PI3K-Akt signaling pathway;>>Proteoglycans in cancer |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBIHC |
| Dilution range | IHC-p1:50-200 ,WB 1:1000-2000 |
| Immunogen | Synthesized peptide derived from human Eif4b AA range: 209-259 |
| Purification | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
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Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | IHC-p1:50-200 | — |
| Mouse | — | — |
| Rat | — | — |
ABMIUM Validated™
IHC
| Species | Dilution | Notes |
|---|
Specifications
Target
eIF4B
Also known as
Eukaryotic translation initiation factor 4B (eIF-4B)
Concentration
1 mg/ml
Working concentration
IHC-p1:50-200 ,WB 1:1000-2000
Purity
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.77% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human; Mouse;Rat
Tested applications
WBIHC
Immunogen
Synthesized peptide derived from human Eif4b AA range: 209-259
Observed band
69kD
Cellular localization
cytosol,polysome,eukaryotic translation initiation factor 4F complex,dendrite,neuronal cell body,
Dilution range
IHC-p1:50-200 ,WB 1:1000-2000
Purification
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Storage & Stability
Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.77% sodium azide.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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