ATG13 Rabbit pAb from ELK Biotechnology Read more ›
ATG13 Rabbit pAb is a research-use antibody from ELK Biotechnology for studying ATG13 in WB, IHC workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
Atg13 was identified as a constitutively expressed protein that was genetically linked to Atg1/Apg1, a protein kinase required for autophagy. Overexpression of Atg1 suppresses the defects in autophagy observed in Atg13 mutants. Autophagy requires a direct association between Atg1 and Atg13, and is inhibited by TOR-dependent phosphorylation of Atg13 under high-nutrient conditions.
| Specificity | This antibody detects endogenous levels of ATG13 at Human, Mouse,Rat |
|---|---|
| Validation evidence captions | Western blot analysis of 1)Hela Cell, 2) Rat Brain Tissue, 3) Mouse Brainr Tissue Lysate using Rabbit pAb diluted at 1:2,000. | Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min). |
| Collaborator | ELK Biotechnology |
| Partner model | ES20862 |
| Target | ATG13 |
| Research areas | >>Autophagy - other, >>Autophagy - animal, >>Longevity regulating pathway, >>Alzheimer disease, >>Amyotrophic lateral sclerosis, >>Huntington disease, >>Spinocerebellar ataxia, >>Pathways of neurodegeneration - multiple diseases |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC |
| Conjugation | Unconjugated |
| Dilution range | IHC-p1:50-200 ,WB 1:1000-2000 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Synthesized peptide derived from human ATG13 AA range: 182-232 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Assay type | Antibody validation/application |
| Purity | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | IHC-p1:50-200 | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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