ABM97079
ATG13 Rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
ATG13 Rabbit pAb from ELK Biotechnology Read more ›
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Product Description
ATG13 Rabbit pAb is an ELK Biotechnology antibody for research workflows involving ATG13.
Background
Atg13 was identified as a constitutively expressed protein that was genetically linked to Atg1/Apg1, a protein kinase required for autophagy. Overexpression of Atg1 suppresses the defects in autophagy observed in Atg13 mutants. Autophagy requires a direct association between Atg1 and Atg13, and is inhibited by TOR-dependent phosphorylation of Atg13 under high-nutrient conditions.
Additional antibody information
| Specificity | This antibody detects endogenous levels of ATG13 at Human, Mouse,Rat |
|---|---|
| Validation evidence captions | Western blot analysis of 1)Hela Cell, 2) Rat Brain Tissue, 3) Mouse Brainr Tissue Lysate using Rabbit pAb diluted at 1:2,000. | Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min). |
Key Facts
Product type
Primary Antibody›
Target: ATG13
| Target | ATG13 |
| Also known as | Autophagy-related protein 13 |
| Concentration | 1 mg/ml |
| Working concentration | IHC-p1:50-200 ,WB 1:1000-2000 |
| Species reactivity | Human; Mouse;Rat |
| Observed band | 72kD |
| Cellular localization | Cytoplasm, cytosol . Preautophagosomal structure . Under starvation conditions, is localized to puncate structures primarily representing the isolation membrane; the isolation membrane sequesters a portion of the cytoplasm resulting in autophagosome formation. . |
| Purity | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.19% sodium azide. |
| Research area | >>Autophagy - other;>>Autophagy - animal;>>Longevity regulating pathway;>>Alzheimer disease;>>Amyotrophic lateral sclerosis;>>Huntington disease;>>Spinocerebellar ataxia;>>Pathways of neurodegeneration - multiple diseases |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBIHC |
| Dilution range | IHC-p1:50-200 ,WB 1:1000-2000 |
| Immunogen | Synthesized peptide derived from human ATG13 AA range: 182-232 |
| Purification | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
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Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | IHC-p1:50-200 | — |
| Mouse | — | — |
| Rat | — | — |
ABMIUM Validated™
IHC
| Species | Dilution | Notes |
|---|
Specifications
Target
ATG13
Also known as
Autophagy-related protein 13
Concentration
1 mg/ml
Working concentration
IHC-p1:50-200 ,WB 1:1000-2000
Purity
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.19% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human; Mouse;Rat
Tested applications
WBIHC
Immunogen
Synthesized peptide derived from human ATG13 AA range: 182-232
Observed band
72kD
Cellular localization
Cytoplasm, cytosol . Preautophagosomal structure . Under starvation conditions, is localized to puncate structures primarily representing the isolation membrane; the isolation membrane sequesters a portion of the cytoplasm resulting in autophagosome formation. .
Dilution range
IHC-p1:50-200 ,WB 1:1000-2000
Purification
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Storage & Stability
Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.19% sodium azide.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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