Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM97057

CaVα2δ3 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

CaVα2δ3 rabbit pAb from ELK Biotechnology Read more ›

CaVα2δ3 rabbit pAb 50µL/100µL
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CaVα2δ3 rabbit pAb 50µL/100µL

Product Description

CaVα2δ3 rabbit pAb is a research-use antibody from ELK Biotechnology for studying CaVα2δ3 in WB workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

calcium voltage-gated channel auxiliary subunit alpha2delta 3(CACNA2D3) Homo sapiens This gene encodes a member of the alpha-2/delta subunit family, a protein in the voltage-dependent calcium channel complex. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization and consist of a complex of alpha-1, alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio. Various versions of each of these subunits exist, either expressed from similar genes or the result of alternative splicing. Research on a highly similar protein in rabbit suggests the protein described in this record is cleaved into alpha-2 and delta subunits. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq, Jul 2008],

Additional antibody information

Specificity CaVα2δ3 protein(A210) detects endogenous levels of CaVα2δ3
Validation evidence captions Western blot analysis of 1) Mouse Brain Tissue, 2)Human Brain Tissue, with CaVα2δ3 Rabbit pAb diluted at 1:2,000.

Key Facts

Product typePrimary Antibody Target: CaVα2δ3
Collaborator ELK Biotechnology
Partner model ES20791
Target CaVα2δ3
Research areas>>MAPK signaling pathway, >>Cardiac muscle contraction, >>Adrenergic signaling in cardiomyocytes, >>Oxytocin signaling pathway, >>Hypertrophic cardiomyopathy, >>Arrhythmogenic right ventricular cardiomyopathy, >>Dilated cardiomyopathy
Reactivity Human,Rat,Mouse
Applications WB
ConjugationUnconjugated
Dilution rangeWB 1:1000-2000
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthetic Peptide of CaVα2δ3 AA range: 500-580
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen.
Assay type Antibody validation/application
Expression system Only detected in brain. Not present in lung, testis, aorta, spleen, jejunum, ventricular muscle and kidney (at protein level). According to PubMed:11687876, it is brain-specific, while according to PubMed:11245980, it is widely expressed.
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:1000-2000
Rat
Mouse

Specifications

Target
CaVα2δ3
Also known as
Voltage-dependent calcium channel subunit alpha-2/delta-3 (Voltage-gated calcium channel subunit alpha-2/delta-3) Cleaved into: Voltage-dependent calcium channel subunit alpha-2-3; Voltage-dependent calcium channel subunit delta-3
Concentration
1 mg/ml
Working concentration
WB 1:1000-2000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Rat,Mouse
Tested applications
WB
Immunogen
Synthetic Peptide of CaVα2δ3 AA range: 500-580
Observed band
120kD
Cellular localization
Membrane ; Single-pass type I membrane protein .
Dilution range
WB 1:1000-2000
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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