ABM97055
CK18 rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
CK18 rabbit pAb from ELK Biotechnology Read more ›
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Product Description
CK18 rabbit pAb is an ELK Biotechnology antibody for research workflows involving Cytokeratin 18.
Background
KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008],
Additional antibody information
| Specificity | The antibody detects endogenous Cytokeratin 18 protein. |
|---|---|
| Validation evidence captions | Western blot analysis of Hela, Rabbit pAb diluted at 1:2000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 |
Key Facts
Product type
Primary Antibody›
Target: Cytokeratin 18
| Target | Cytokeratin 18 |
| Also known as | KRT18; CYK18; PIG46; Keratin, type I cytoskeletal 18; Cell proliferation-inducing gene 46 protein; Cytokeratin-18; CK-18; Keratin-18; K18 |
| Working concentration | WB: 1:1000-2000 |
| Species reactivity | Human |
| Observed band | 46kD |
| Cellular localization | Cytoplasm, perinuclear region. Nucleus, nucleolus. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen. |
| Form / buffer | PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol. |
| Research area | >>Estrogen signaling pathway;>>Staphylococcus aureus infection |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WB |
| Dilution range | WB: 1:1000-2000 |
| Immunogen | Recombinant Protein of Keratin type I cytoskeletal 18 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen. |
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Reactivity & Application Validation
1 species| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | WB: 1:1000-2000 | — |
Specifications
Target
Cytokeratin 18
Also known as
KRT18; CYK18; PIG46; Keratin, type I cytoskeletal 18; Cell proliferation-inducing gene 46 protein; Cytokeratin-18; CK-18; Keratin-18; K18
Working concentration
WB: 1:1000-2000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen.
Form / buffer
PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human
Tested applications
WB
Immunogen
Recombinant Protein of Keratin type I cytoskeletal 18
Observed band
46kD
Cellular localization
Cytoplasm, perinuclear region. Nucleus, nucleolus.
Dilution range
WB: 1:1000-2000
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen.
Storage & Stability
Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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